The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionRegulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1. Modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury.
Tissue specificityHighly expressed in skeletal muscle, heart, kidney and pancreas. Expressed in choroid, retina and epidermal melanocytes. Expressed in eye muscles and thyroid follicular cells.
Sequence similaritiesContains 6 ANK repeats.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton. Expressed diffusely in cytoplasm.
Uveal autoantigen with coiled coil domains and ankyrin repeats antibody
Uveal autoantigen with coiled-coil domains and ankyrin repeats antibody
Anti-UACA antibody images
Immunoprecipitation - UACA antibody (ab99320)
Detection of UACA in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99320 at 3 µg/mg lysate for IP. ab99323, at 1 µg/ml, was used for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 3 seconds.
References for Anti-UACA antibody (ab99320)
has not yet been referenced specifically in any publications.
Publishing research using ab99320? Please let us know so that we can cite the reference in this datasheet.
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