Overview

  • Product nameAnti-UACA antibody
    See all UACA primary antibodies
  • Description
    Rabbit polyclonal to UACA
  • Tested applicationsSuitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Chimpanzee, Rhesus monkey, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1-50 (MKSLKSRLRR QDVPGPASSG AAAASAHAAD WNKYDDRLMK AAERGDVEKV) of Human UACA (NP_060473.2).

  • Positive control
    • Hela whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab99320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionRegulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1.
      Modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury.
    • Tissue specificityHighly expressed in skeletal muscle, heart, kidney and pancreas. Expressed in choroid, retina and epidermal melanocytes. Expressed in eye muscles and thyroid follicular cells.
    • Sequence similaritiesContains 6 ANK repeats.
    • Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton. Expressed diffusely in cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ10128 antibody
      • KIAA1561 antibody
      • MGC141967 antibody
      • MGC141969 antibody
      • Nuclear membrane binding protein antibody
      • NUCLING antibody
      • UACA antibody
      • UACA_HUMAN antibody
      • Uveal autoantigen with coiled coil domains and ankyrin repeats antibody
      • Uveal autoantigen with coiled-coil domains and ankyrin repeats antibody
      see all

    Anti-UACA antibody images

    • Detection of UACA in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99320 at 3 µg/mg lysate for IP. ab99323, at 1 µg/ml, was used for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 3 seconds.

    References for Anti-UACA antibody (ab99320)

    ab99320 has not yet been referenced specifically in any publications.

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