Overview

  • Product nameAnti-UACA antibody
    See all UACA primary antibodies
  • Description
    Rabbit polyclonal to UACA
  • Tested applicationsSuitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region between residue 675-725 (KAKLAQHVKP EEHEQVKSRL EQKSGELGKK ITELTLKNQT LQKEIEKVYL D) of Human UACA (NP_060473.2).

  • Positive control
    • HeLa whole cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab99321 was affinity purified using an epitope specific to UACA immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P Use at an assay dependent concentration.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionRegulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1.
      Modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury.
    • Tissue specificityHighly expressed in skeletal muscle, heart, kidney and pancreas. Expressed in choroid, retina and epidermal melanocytes. Expressed in eye muscles and thyroid follicular cells.
    • Sequence similaritiesContains 6 ANK repeats.
    • Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton. Expressed diffusely in cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ10128 antibody
      • KIAA1561 antibody
      • MGC141967 antibody
      • MGC141969 antibody
      • Nuclear membrane binding protein antibody
      • NUCLING antibody
      • UACA antibody
      • UACA_HUMAN antibody
      • Uveal autoantigen with coiled coil domains and ankyrin repeats antibody
      • Uveal autoantigen with coiled-coil domains and ankyrin repeats antibody
      see all

    Anti-UACA antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling UACA with ab99321 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of UACA in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99321 at 3 µg/mg lysate for IP. ab99323, at 1 µg/ml, was used for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 3 seconds.

    References for Anti-UACA antibody (ab99321)

    ab99321 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"