Anti-UAP1 antibody (ab95949)
Key features and details
- Rabbit polyclonal to UAP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UAP1 antibody
See all UAP1 primary antibodies -
Description
Rabbit polyclonal to UAP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Zebrafish -
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 53-473 of UAP1 isoform AGX1 (Human)
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Positive control
- H1299 lysate, A549 cells (IF), and ES-2 xenograft (IHC)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P. -
Tissue specificity
Widely expressed. Isoform AGX1 is more abundant in testis than isoform AGX2, while isoform AGX2 is more abundant than isoform AGX1 in somatic tissue. Expressed at low level in placenta, muscle and liver. -
Pathway
Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1. -
Sequence similarities
Belongs to the UDPGP type 1 family. -
Cellular localization
Cytoplasm. In spermatozoa, localized to the principal piece of the tail, the neck region of the head and to a lesser extent, the midpiece of the tail. - Information by UniProt
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Database links
- Entrez Gene: 535325 Cow
- Entrez Gene: 6675 Human
- Entrez Gene: 107652 Mouse
- Omim: 602862 Human
- SwissProt: Q16222 Human
- SwissProt: Q91YN5 Mouse
- Unigene: 492859 Human
- Unigene: 27969 Mouse
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Alternative names
- AGX 1 antibody
- AGX antibody
- AGX-1 antibody
see all
Images
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Anti-UAP1 antibody (ab95949) at 1/3000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 57 kDa
gel concentration: 7.5% -
Immunofluorescence analysis of paraformaldehyde fixed A549 cells using ab95949: (1) at 1/200 dilution, (2) merged with DNA probe.
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Immunohistochemical analysis of paraffin embedded ES 2 xenograft using ab95949 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab95949 has been referenced in 2 publications.
- Feinberg D et al. Inhibition of O-GlcNAcylation Decreases the Cytotoxic Function of Natural Killer Cells. Front Immunol 13:841299 (2022). PubMed: 35479087
- Mason B et al. Fbxl17 is rearranged in breast cancer and loss of its activity leads to increased global O-GlcNAcylation. Cell Mol Life Sci 77:2605-2620 (2020). PubMed: 31560077