1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/10 - 1/100.
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.
Widely expressed. Isoform AGX1 is more abundant in testis than isoform AGX2, while isoform AGX2 is more abundant than isoform AGX1 in somatic tissue. Expressed at low level in placenta, muscle and liver.
Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.
Belongs to the UDPGP type 1 family.
Cytoplasm. In spermatozoa, localized to the principal piece of the tail, the neck region of the head and to a lesser extent, the midpiece of the tail.
Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling UAP1 with purified ab155287 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
Western blot - Anti-UAP1 antibody [EPR10259] (ab155287)
All lanes : Anti-UAP1 antibody [EPR10259] (ab155287) at 1/1000 dilution
Lane 1 : Human testis lysate Lane 2 : Jurkat cell lysate Lane 3 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat anti-rabbit HRP at 1/2000 dilution