Recombinant
RabMAb

Anti-UAP1 antibody [EPR10259] (ab155287)

Overview

  • Product name
    Anti-UAP1 antibody [EPR10259]
    See all UAP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10259] to UAP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide, corresponding to residues in Human UAP1 (UniProt: Q16222).

  • Positive control
    • Human testis, Jurkat and A549 lysates; Human gastric carcinoma tissue; Permeabilized Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/500.

Target

  • Function
    Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.
  • Tissue specificity
    Widely expressed. Isoform AGX1 is more abundant in testis than isoform AGX2, while isoform AGX2 is more abundant than isoform AGX1 in somatic tissue. Expressed at low level in placenta, muscle and liver.
  • Pathway
    Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.
  • Sequence similarities
    Belongs to the UDPGP type 1 family.
  • Cellular localization
    Cytoplasm. In spermatozoa, localized to the principal piece of the tail, the neck region of the head and to a lesser extent, the midpiece of the tail.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGX 1 antibody
    • AGX antibody
    • AGX-1 antibody
    • AGX-2 antibody
    • AGX1 antibody
    • Antigen X antibody
    • AntigenX antibody
    • SPAG 2 antibody
    • SPAG2 antibody
    • Sperm associated antigen 2 antibody
    • Sperm-associated antigen 2 antibody
    • UAP 1 antibody
    • uap1 antibody
    • UAP1_HUMAN antibody
    • UDP N acetylhexosamine pyrophosphorylase antibody
    • UDP N acteylglucosamine pyrophosphorylase 1 antibody
    • UDP-N-acetylglucosamine pyrophosphorylase antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling UAP1 with purified ab155287 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • All lanes : Anti-UAP1 antibody [EPR10259] (ab155287) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 59 kDa

  • Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling UAP1 with ab155287 at 1/50 dilution.
  • Flow cytometric analysis of permeabilized Jurkat cells labeling UAP1 with ab155287 at 1/100 dilution (red) compared with a rabbit IgG negative control (green).
  • Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab155287 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab155287 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155287 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab155287 showing +ve staining.

References

This product has been referenced in:
  • Zahreddine HA  et al. The eukaryotic translation initiation factor eIF4E harnesses hyaluronan production to drive its malignant activity. Elife 6:N/A (2017). Read more (PubMed: 29111978) »
  • Chaveroux C  et al. Nutrient shortage triggers the hexosamine biosynthetic pathway via the GCN2-ATF4 signalling pathway. Sci Rep 6:27278 (2016). WB . Read more (PubMed: 27255611) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HBEC)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Treatment
glucose deprivation
Specification
HBEC
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Cedric Chaveroux

Verified customer

Submitted Jun 08 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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