Overview

  • Product nameAnti-UAP56 antibody
    See all UAP56 primary antibodies
  • Description
    Rabbit polyclonal to UAP56
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human UAP56.

    (Peptide available as ab48225.)

  • Positive control
    • ab47955 gave a positive signal in the following Whole Cell Lysates: Hela, Jurkat, HepG2 and A431. It also gave a positive signal in human tonsil tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab47955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionComponent of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
    Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DECD subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • DomainThe helicase C-terminal domain mediates interaction with THOC4.
  • Cellular localizationNucleus. Nucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0610030D10Rik antibody
    • 4F2-LC6 antibody
    • 56 kDa U2AF65-associated protein antibody
    • AI428441 antibody
    • ATP-dependent RNA helicase p47 antibody
    • B(0,+)-type amino acid transporter 1 antibody
    • BAT1 antibody
    • Bat1a antibody
    • D17H6S81E antibody
    • D17H6S81E-1 antibody
    • D6S81E antibody
    • D6S81Eh antibody
    • DDX39B antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B antibody
    • DEAD box protein UAP56 antibody
    • DX39B_HUMAN antibody
    • EC 3.6.1.- antibody
    • Glycoprotein-associated amino acid transporter b0,+AT1 antibody
    • HLA-B-associated transcript 1 protein antibody
    • HLA-B-associated transcript 1A antibody
    • HLA-B-associated transcript-1 antibody
    • MGC127051 antibody
    • MGC19235 antibody
    • MGC38799 antibody
    • nuclear RNA helicase (DEAD family) antibody
    • OTTHUMP00000029229 antibody
    • OTTHUMP00000165889 antibody
    • OTTHUMP00000165890 antibody
    • p47 antibody
    • Solute carrier family 7 member 9 antibody
    • Spliceosome RNA helicase BAT1 antibody
    • Spliceosome RNA helicase DDX39B antibody
    • U2AF65-associayed protein, 56-KD antibody
    • UAP56 antibody
    see all

Anti-UAP56 antibody images

  • All lanes : Anti-UAP56 antibody (ab47955) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • ICC/IF image of ab47955 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab47955, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • IHC image of ab47955 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47955, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-UAP56 antibody (ab47955)

This product has been referenced in:
  • Read EK & Digard P Individual influenza A virus mRNAs show differential dependence on cellular NXF1/TAP for their nuclear export. J Gen Virol 91:1290-301 (2010). WB, In situ hybridization ; Human . Read more (PubMed: 20071484) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK 293)
Loading amount 25 µg
Specification HEK 293
Gel Running Conditions Reduced Denaturing (10 % PAGE)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Apr 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"