Overview

  • Product nameAnti-UBA5 antibody
    See all UBA5 primary antibodies
  • Description
    Mouse polyclonal to UBA5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human) (NP_079094.1)

  • Positive control
    • Human colon tissue lysate; UBA5 transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).

Target

  • FunctionE1-like enzyme which activates UFM1 and SUMO2.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the ubiquitin-activating E1 family. UBA5 subfamily.
  • Cellular localizationCytoplasm. Nucleus. Localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SUMO2.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5730525G14Rik antibody
    • AW240750 antibody
    • FLJ23251 antibody
    • FLJ23251UBA5 antibody
    • MGC105565 antibody
    • ThiFP1 antibody
    • UBA5 antibody
    • UBA5 ubiquitin activating enzyme E1 homolog antibody
    • UBA5_HUMAN antibody
    • UBE1DC antibody
    • UBE1DC1 antibody
    • Ubiquitin activating enzyme 5 antibody
    • Ubiquitin activating enzyme E1 domain containing 1 antibody
    • Ubiquitin activating enzyme E1 domain containing protein 1 antibody
    • Ubiquitin like modifier activating enzyme 5 antibody
    • Ubiquitin-activating enzyme 5 antibody
    • Ubiquitin-activating enzyme E1 domain-containing protein 1 antibody
    • Ubiquitin-like modifier-activating enzyme 5 antibody
    • UFM1 activating enzyme antibody
    • UFM1-activating enzyme antibody
    see all

Anti-UBA5 antibody images

  • Anti-UBA5 antibody (ab69046) at 1/500 dilution + Human colon tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 45 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-UBA5 antibody (ab69046) at 1/500 dilution

    Lane 1 : UBA5 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 45 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-UBA5 antibody (ab69046)

ab69046 has not yet been referenced specifically in any publications.

Product Wall

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