Overview

  • Product name
  • Description
    Rabbit polyclonal to UBA5
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal region of human UBA5.

  • Positive control
    • Extracts from HepG2 and 293 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 45 kDa.
ELISA 1/5000.

Target

  • Function
    E1-like enzyme which activates UFM1 and SUMO2.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the ubiquitin-activating E1 family. UBA5 subfamily.
  • Cellular localization
    Cytoplasm. Nucleus. Localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SUMO2.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5730525G14Rik antibody
    • AW240750 antibody
    • FLJ23251 antibody
    • FLJ23251UBA5 antibody
    • MGC105565 antibody
    • ThiFP1 antibody
    • UBA5 antibody
    • UBA5 ubiquitin activating enzyme E1 homolog antibody
    • UBA5_HUMAN antibody
    • UBE1DC antibody
    • UBE1DC1 antibody
    • Ubiquitin activating enzyme 5 antibody
    • Ubiquitin activating enzyme E1 domain containing 1 antibody
    • Ubiquitin activating enzyme E1 domain containing protein 1 antibody
    • Ubiquitin like modifier activating enzyme 5 antibody
    • Ubiquitin-activating enzyme 5 antibody
    • Ubiquitin-activating enzyme E1 domain-containing protein 1 antibody
    • Ubiquitin-like modifier-activating enzyme 5 antibody
    • UFM1 activating enzyme antibody
    • UFM1-activating enzyme antibody
    see all

Images

  • All lanes : Anti-UBA5 antibody (ab74372) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : 293 cell extract
    Lane 3 : 293 cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

References

ab74372 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab74372.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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