Overview

  • Product name
  • Description
    Rabbit polyclonal to UBA52
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the C-terminal region of Human Ubiquitin(EDGRTLSDYNIQKEST).

  • Positive control
    • Human Prostate and Testis tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab104455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 15 kDa.
ELISA 1/50 - 1/100.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBA52 is a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.
  • Cellular localization
    Ubiquitin: Cytoplasm. Nucleus. 60S ribosomal protein L40: Cytoplasm
  • Database links
  • Alternative names
    • 60S ribosomal protein L40 antibody
    • CEP52 antibody
    • HUBCEP52 antibody
    • L40 antibody
    • MGC126879 antibody
    • MGC57125 antibody
    • RPL40 antibody
    • UBA 52 antibody
    • Ubiquitin 52 amino acid fusion protein antibody
    • Ubiquitin 60S ribosomal protein L40 antibody
    • Ubiquitin A 52 residue ribosomal protein fusion product 1 antibody
    • Ubiquitin carboxyl extension protein 52 antibody
    • Ubiquitin CEP52 antibody
    see all

Images

  • ab104455, at 10 µg/ml, staining UBA52 in formalin-fixed, paraffin-embedded Human Prostate by Immunohistochemistry, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab104455, at 10 µg/ml, staining UBA52 in formalin-fixed, paraffin-embedded Human Testis by Immunohistochemistry, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.


  • Predicted band size : 15 kDa

References

ab104455 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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