Recombinant
RabMAb

Anti-UBA52 antibody [EPR4546] (ab109227)

Overview

  • Product name
    Anti-UBA52 antibody [EPR4546]
    See all UBA52 primary antibodies
  • Description
    Rabbit monoclonal [EPR4546] to UBA52
  • Tested applications
    Suitable for: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from the N-terminus of Human UBA52.

  • Positive control
    • Fetal kidney, Human placenta, 293T and JAR cell lysates. Human brain tissue. SH-SY-5Y cells. FC: HepG2 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2500.
WB 1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.

Target

  • Relevance
    Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBA52 is a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.
  • Cellular localization
    Ubiquitin: Cytoplasm. Nucleus. 60S ribosomal protein L40: Cytoplasm
  • Database links
  • Alternative names
    • 60S ribosomal protein L40 antibody
    • CEP52 antibody
    • HUBCEP52 antibody
    • L40 antibody
    • MGC126879 antibody
    • MGC57125 antibody
    • RPL40 antibody
    • UBA 52 antibody
    • Ubiquitin 52 amino acid fusion protein antibody
    • Ubiquitin 60S ribosomal protein L40 antibody
    • Ubiquitin A 52 residue ribosomal protein fusion product 1 antibody
    • Ubiquitin carboxyl extension protein 52 antibody
    • Ubiquitin CEP52 antibody
    see all

Anti-UBA52 antibody [EPR4546] images

  • All lanes : Anti-UBA52 antibody [EPR4546] (ab109227) at 1/1000 dilution

    Lane 1 : fetal kidney lysates
    Lane 2 : Human placenta lysates
    Lane 3 : 293T cell lysates
    Lane 4 : JAR cell lysates

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 15 kDa
    Observed band size : 10 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded Human brain tissue using 1/500 ab109227.
  • Flow cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling UBA52 (red) with purified ab109227 at a 1/2500 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunofluorescent staining of SH-SY-5Y cells using ab109227 at 1/250

References for Anti-UBA52 antibody [EPR4546] (ab109227)

This product has been referenced in:
  • Francis C  et al. Divergence of RNA localization between rat and mouse neurons reveals the potential for rapid brain evolution. BMC Genomics 15:883 (2014). ICC ; Mouse, Rat . Read more (PubMed: 25301173) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry.

I am sorry to confirm that the cross-reactivity of the ab109227 UBA52 antibody to other Ubiquitin proteins have not been verified by testing. However, based on sequence analysis, the immunogen does not share seque...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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