Synthetic peptide from the N-terminus of Human UBA52.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Our Abpromise guarantee covers the use of ab109227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 15 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/500 - 1/1000. Perform antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
Flow cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling UBA52 (red) with purified ab109227 at a 1/2500 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).