Overview

  • Product nameAnti-UBA52 antibody [EPR4547]
    See all UBA52 primary antibodies
  • Description
    Rabbit monoclonal [EPR4547] to UBA52
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human UBA52.

  • Positive control
    • WB: Human placenta, Raji, fetal kidney, 293T and Jurkat lysates IHC-P: Human brain tissue IF/ICC: HepG2 cell line.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 15 kDa).
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
ICC/IF 1/250 - 1/500.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • RelevanceUbiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBA52 is a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.
    • Cellular localizationUbiquitin: Cytoplasm. Nucleus. 60S ribosomal protein L40: Cytoplasm
    • Database links
    • Alternative names
      • 60S ribosomal protein L40 antibody
      • CEP52 antibody
      • HUBCEP52 antibody
      • L40 antibody
      • MGC126879 antibody
      • MGC57125 antibody
      • RPL40 antibody
      • UBA 52 antibody
      • Ubiquitin 52 amino acid fusion protein antibody
      • Ubiquitin 60S ribosomal protein L40 antibody
      • Ubiquitin A 52 residue ribosomal protein fusion product 1 antibody
      • Ubiquitin carboxyl extension protein 52 antibody
      • Ubiquitin CEP52 antibody
      see all

    Anti-UBA52 antibody [EPR4547] images

    • ab109230 stained HepG2 cells. The cells were 4%  formaldehdye fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109230 at 1/200) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab96899 Dylight 488 goat anti-rabbit IgG used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • All lanes : Anti-UBA52 antibody [EPR4547] (ab109230) at 1/1000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : Raji cell lysate
      Lane 3 : Fetal kidney lysate
      Lane 4 : 293T cell lysate
      Lane 5 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 15 kDa
      Observed band size : 10 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of UBA52 in paraffin embedded Human brain tissue, using ab109230 at a 1/500 dilution.

    References for Anti-UBA52 antibody [EPR4547] (ab109230)

    ab109230 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"