Overview

  • Product nameAnti-Uba6 antibody
    See all Uba6 primary antibodies
  • Description
    Mouse monoclonal to Uba6
  • Tested applicationsSuitable for: WB, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment corresponding to amino acids 962-1052 of Human Uba6 with proprietary 26 kDa tag (NP_060697).

  • Positive control
    • Human testis tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab118344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 36.01 kDa (predicted molecular weight: 118 kDa). This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 10 µg/ml.

Target

  • FunctionActivates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.
  • Tissue specificityWidely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • E1-L2 antibody
    • FLJ10808 antibody
    • FLJ23367 antibody
    • Monocyte protein 4 antibody
    • MOP 4 antibody
    • MOP-4 antibody
    • MOP4 antibody
    • UBA 6 antibody
    • Uba6 antibody
    • UBA6 ubiquitin activating enzyme E1 antibody
    • UBA6_HUMAN antibody
    • UBE1L2 antibody
    • Ubiquitin activating enzyme 6 antibody
    • Ubiquitin activating enzyme E1 like 2 antibody
    • Ubiquitin activating enzyme E1 like protein 2 antibody
    • Ubiquitin like modifier activating enzyme 6 antibody
    • Ubiquitin-activating enzyme 6 antibody
    • Ubiquitin-activating enzyme E1-like protein 2 antibody
    • Ubiquitin-like modifier-activating enzyme 6 antibody
    see all

Anti-Uba6 antibody images

  • Anti-Uba6 antibody (ab118344) at 5 µg/ml + Immunogen peptide at 0.2 µg

    Predicted band size : 118 kDa
  • ab118344, at 3 µg/ml staining Uba6 in formalin-fixed, paraffin-embedded Human testis by Immunohistochemistry.
  • Detection limit for ab118344 is 1 ng/ml as a capture antibody.

References for Anti-Uba6 antibody (ab118344)

ab118344 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"