Overview

  • Product name
  • Description
    Rabbit polyclonal to Uba6
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A portion of amino acids 775-825 of human Uba6 (NP_060697).

  • Positive control
    • Daudi and RAW cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab72091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects bands of approximately 44 and 118 kDa (predicted molecular weight: 118 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.
    • Tissue specificity
      Widely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the ubiquitin-activating E1 family.
    • Information by UniProt
    • Database links
    • Alternative names
      • E1-L2 antibody
      • FLJ10808 antibody
      • FLJ23367 antibody
      • Monocyte protein 4 antibody
      • MOP 4 antibody
      • MOP-4 antibody
      • MOP4 antibody
      • UBA 6 antibody
      • Uba6 antibody
      • UBA6 ubiquitin activating enzyme E1 antibody
      • UBA6_HUMAN antibody
      • UBE1L2 antibody
      • Ubiquitin activating enzyme 6 antibody
      • Ubiquitin activating enzyme E1 like 2 antibody
      • Ubiquitin activating enzyme E1 like protein 2 antibody
      • Ubiquitin like modifier activating enzyme 6 antibody
      • Ubiquitin-activating enzyme 6 antibody
      • Ubiquitin-activating enzyme E1-like protein 2 antibody
      • Ubiquitin-like modifier-activating enzyme 6 antibody
      see all

    Images

    • Lanes 1 - 2 : Anti-Uba6 antibody (ab72091) at 1 µg/ml
      Lane 3 : Anti-Uba6 antibody (ab72091) at 0.5 µg/ml

      Lane 1 : Daudi cell lysate
      Lane 2 : Daudi cell lysate plus immunizing peptide
      Lane 3 : RAW cell lysate


      Predicted band size : 118 kDa
      Observed band size : 118 kDa
      Additional bands at : 44 kDa. We are unsure as to the identity of these extra bands.

    References

    ab72091 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab72091.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up