Overview

  • Product nameAnti-Uba6 antibody
    See all Uba6 primary antibodies
  • Description
    Rabbit polyclonal to Uba6
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Cow, Human, Chimpanzee
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 300-350 of Human Uba6

  • Positive control
    • Brain cortex tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab78053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 118 kDa.
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionActivates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.
  • Tissue specificityWidely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • E1-L2 antibody
    • FLJ10808 antibody
    • FLJ23367 antibody
    • Monocyte protein 4 antibody
    • MOP 4 antibody
    • MOP-4 antibody
    • MOP4 antibody
    • UBA 6 antibody
    • Uba6 antibody
    • UBA6 ubiquitin activating enzyme E1 antibody
    • UBA6_HUMAN antibody
    • UBE1L2 antibody
    • Ubiquitin activating enzyme 6 antibody
    • Ubiquitin activating enzyme E1 like 2 antibody
    • Ubiquitin activating enzyme E1 like protein 2 antibody
    • Ubiquitin like modifier activating enzyme 6 antibody
    • Ubiquitin-activating enzyme 6 antibody
    • Ubiquitin-activating enzyme E1-like protein 2 antibody
    • Ubiquitin-like modifier-activating enzyme 6 antibody
    see all

Anti-Uba6 antibody images

  • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded brain cortex tissue using ab78053 at a concentration of 10 ?g/ml.

References for Anti-Uba6 antibody (ab78053)

ab78053 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 30000 cells
Gel Running Conditions Reduced Denaturing (6% gel)
Sample Chinese Hamster Cell lysate - whole cell (CHO-K1 cell lysate)
Specification CHO-K1 cell lysate
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
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Verified customer

Submitted Aug 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step PBS containing 40 mM glycine, 1% bovine serum albumin, and 0.2% gelatin as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 26°C
Sample Chinese Hamster Cell (CHO-K1)
Specification CHO-K1
Permeabilization Yes - 0.2% Triton X-100 in PBS
Fixative Paraformaldehyde
Username

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Verified customer

Submitted Aug 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"