Overview

  • Product name
  • Description
    Rabbit polyclonal to UBAP2L
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between residues 400 and 450 of human UBAP2L (AAH03170.1).

  • Positive control
    • HeLa whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at a concentration of 2 - 5 µg/mg.
    WB: 1/5000 - 1/25000. Detects a band of approximately 160 kDa (predicted molecular weight: 115 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Contains 1 UBA domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      Acetylated.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ42300 antibody
      • KIAA0144 antibody
      • NICE 4 antibody
      • NICE4 antibody
      • Protein NICE-4 antibody
      • Protein NICE4 antibody
      • Ubap2l antibody
      • Ubiquitin associated protein 2 like antibody
      • Ubiquitin-associated protein 2-like antibody
      • UBP2L_HUMAN antibody
      see all

    Images

    • All lanes : Anti-UBAP2L antibody (ab70319) at 0.03 µg/ml

      Lane 1 : Mock treated Hela cell lysate
      Lane 2 : Hela cell lysate treated with a control siRNA
      Lane 3 : Hela cell lysate treated with siRNA for UBAP2L

      Lysates/proteins at 100 µg per lane.


      Predicted band size : 115 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:
    • Zhao B  et al. Downregulation of ubiquitin-associated protein 2-like with a short hairpin RNA inhibits human glioma cell growth in vitro. Int J Mol Med 36:1012-8 (2015). WB . Read more (PubMed: 26310274) »
    • Li D & Huang Y Knockdown of ubiquitin associated protein 2-like inhibits the growth and migration of prostate cancer cells. Oncol Rep 32:1578-84 (2014). Read more (PubMed: 25069639) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab70319.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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