Overview

  • Product name
  • Description
    Rabbit polyclonal to UBASH3A
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 434-483 (PCSLPRRSRG IKDFENDPPL SSCGIFQSRI AGDALLDSGI RISSVFASPA) of Human UBASH3A (NP_061834).

  • Positive control
    • Human Placenta lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 74 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. Exhibits negligigle protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain.
  • Tissue specificity
    Highest expression of UBASH3A in tissues belonging to the immune system, including spleen, peripheral blood leukocytes, thymus and bone marrow.
  • Sequence similarities
    Contains 1 SH3 domain.
    Contains 1 UBA domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cbl interacting protein 4 antibody
    • Cbl-interacting protein 4 antibody
    • CLIP4 antibody
    • STS 2 antibody
    • STS-2 antibody
    • Suppressor of T cell receptor signaling 2 antibody
    • Suppressor of T-cell receptor signaling 2 antibody
    • T cell ubiquitin ligand antibody
    • T cell ubiquitin ligand protein antibody
    • T-cell ubiquitin ligand 1 antibody
    • TULA antibody
    • TULA-1 antibody
    • UBASH3A antibody
    • ubiquitin associated and SH3 domain containing, A antibody
    • Ubiquitin-associated and SH3 domain-containing protein A antibody
    • UBS3A_HUMAN antibody
    see all

Images

  • Anti-UBASH3A antibody (ab105058) at 1 µg/ml + Human Placenta Lysate at 10 µg

    Predicted band size : 74 kDa

References

This product has been referenced in:
  • Hu H  et al. Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination. J Exp Med 213:399-414 (2016). WB ; Mouse . Read more (PubMed: 26903241) »

See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (thymocytes peripheral CD4 T cells)
Gel Running Conditions
Reduced Denaturing (7-15%)
Loading amount
2e+006 cells
Treatment
pervanadate
Specification
thymocytes peripheral CD4 T cells
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Gaetan Blaize

Verified customer

Submitted Jun 26 2017

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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