Overview

  • Product nameAnti-UBC3B antibody
    See all UBC3B primary antibodies
  • Description
    Goat polyclonal to UBC3B
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, Electron Microscopymore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide: AQQQMTSSQKALM, corresponding to N terminal amino acids 2-14 of Human UBC3B.

  • General notesPrincipal Names – UBE2R2; UBC3B; CDC34B; FLJ20419; MGC10481; ubiquitin-conjugating enzyme E2R 2; ubiquitin-conjugating enzyme UBC3B. Official Gene Symbol - UBE2R2. GenBank Accession Number – NP_060281. LocusLink ID - 54926 (human); 67615 (mouse). Gene Ontology terms - ubiquitin conjugating enzyme activity; ubiquitin-protein ligase activity; ubiquitin cycle; protein modification; ligase activity. An Ubiquitin-conjugating enzyme.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab4281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Can be blocked with Human UBC3B peptide (ab23017). No signal obtained yet but low background observed in Human Testis and U937 lysates at up to 1 µg/ml.
ELISA Use at an assay dependent concentration. antibody detection limit dilution 1/4000.
Electron Microscopy Use at an assay dependent concentration. This product stained chromatoid bodies in Rat spermatids.

Target

  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination. May be involved in degradation of katenin.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC34B antibody
    • E2 CDC34B antibody
    • FLJ20419 antibody
    • MGC10481 antibody
    • UB2R2 antibody
    • UB2R2_HUMAN antibody
    • UBE2R2 antibody
    • Ubiquitin carrier protein R2 antibody
    • Ubiquitin conjugating enzyme E2 CDC34B antibody
    • Ubiquitin conjugating enzyme E2 R2 antibody
    • Ubiquitin conjugating enzyme E2R 2 antibody
    • Ubiquitin conjugating enzyme UBC3B antibody
    • Ubiquitin protein ligase R2 antibody
    • Ubiquitin-conjugating enzyme E2 R2 antibody
    • Ubiquitin-conjugating enzyme E2-CDC34B antibody
    • Ubiquitin-protein ligase R2 antibody
    see all

Anti-UBC3B antibody images

  • ab4281 (2µg/ml) shows staining of paraffin embedded Human Testis stains spermatocytes. Steamed antigen retrieval with citrate buffer pH 6 carried out and HRP-staining.

References for Anti-UBC3B antibody (ab4281)

This product has been referenced in:
  • Haraguchi CM  et al. Chromatoid bodies: aggresome-like characteristics and degradation sites for organelles of spermiogenic cells. J Histochem Cytochem 53:455-65 (2005). Electron Microscopy ; Rat . Read more (PubMed: 15805420) »

See 1 Publication for this product

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Thank you for your enquiry. As you are probably aware this product is a Fast-Track antibody and it is not fully characterized. Please take a look a the datasheet and read the Terms and Conditions for Fast Track antibodies We have not tested it ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"