Our Abpromise guarantee covers the use of ab4281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Can be blocked with Human UBC3B peptide (ab23017). No signal obtained yet but low background observed in Human Testis and U937 lysates at up to 1 µg/ml.|
|ELISA||Use at an assay dependent concentration. antibody detection limit dilution 1/4000.|
|Electron Microscopy||Use at an assay dependent concentration. This product stained chromatoid bodies in Rat spermatids.|
ab4281 (2µg/ml) shows staining of paraffin embedded Human Testis stains spermatocytes. Steamed antigen retrieval with citrate buffer pH 6 carried out and HRP-staining.