Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab36980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications
    ISGylation suppresses ubiquitin E2 enzyme activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • UB2E1_HUMAN antibody
    • UbcH6 antibody
    • Ube2e1 antibody
    • Ubiquitin carrier protein E1 antibody
    • Ubiquitin conjugating enzyme E2E 1 (homologous to yeast UBC4/5) antibody
    • Ubiquitin conjugating enzyme E2E 1 antibody
    • Ubiquitin-conjugating enzyme E2 E1 antibody
    • ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast) antibody
    • Ubiquitin-protein ligase E1 antibody
    see all

Anti-UBCH6/UBE2E1 antibody images

  • Anti-UBCH6/UBE2E1 antibody (ab36980) at 1 µg/ml + HUVEC whole cell lysate at 20 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • UBCH6/UBE2E1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to UBCH6/UBE2E1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab36980.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 25kDa: hnRNP A1; non specific - 18kDa: We are unsure as to the identity of this extra band.
  • ICC/IF image of ab36980 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab36980, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

References for Anti-UBCH6/UBE2E1 antibody (ab36980)

This product has been referenced in:
  • Hans F  et al. UBE2E ubiquitin-conjugating enzymes and ubiquitin isopeptidase Y regulate TDP-43 protein ubiquitination. J Biol Chem 289:19164-79 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24825905) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"