Overview

  • Product name
    Anti-UBCH6/UBE2E1 antibody
    See all UBCH6/UBE2E1 primary antibodies
  • Description
    Rabbit polyclonal to UBCH6/UBE2E1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 72 - 121 (KGDNIYEWRS TILGPPGSVY EGGVFFLDIT FTPEYPFKPP KVTFRTRIYH) of Human UBCH6/UBE2E1 (NP_003332)

  • Positive control
    • COLO205 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications
    ISGylation suppresses ubiquitin E2 enzyme activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • UB2E1_HUMAN antibody
    • UbcH6 antibody
    • Ube2e1 antibody
    • Ubiquitin carrier protein E1 antibody
    • Ubiquitin conjugating enzyme E2E 1 (homologous to yeast UBC4/5) antibody
    • Ubiquitin conjugating enzyme E2E 1 antibody
    • Ubiquitin-conjugating enzyme E2 E1 antibody
    • ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast) antibody
    • Ubiquitin-protein ligase E1 antibody
    see all

Images

  • Anti-UBCH6/UBE2E1 antibody (ab90066) at 1 µg/ml + COLO205 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 21 kDa

References

This product has been referenced in:
  • Hoedt E  et al. SILAC-based proteomic profiling of the human MDA-MB-231 metastatic breast cancer cell line in response to the two antitumoral lactoferrin isoforms: the secreted lactoferrin and the intracellular delta-lactoferrin. PLoS One 9:e104563 (2014). WB ; Human . Read more (PubMed: 25116916) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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