Overview

  • Product name
  • Description
    Rabbit polyclonal to UBE1C
  • Specificity
    This antibody is specific for UBE1C (C term).
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide (10-30 aa in length) at the C terminal of last 50 amino acids of UBE1C (Human)

  • Positive control
    • Mouse brain tissue lysate and human hepatocarcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody was eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 52 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
  • Tissue specificity
    Ubiquitously expressed.
  • Pathway
    Protein modification; protein neddylation.
  • Sequence similarities
    Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp566J164 antibody
    • EC 6.3.2. antibody
    • hUba3 antibody
    • MGC22384 antibody
    • NEDD8 activating enzyme E1 catalytic subunit antibody
    • NEDD8 activating enzyme E1C antibody
    • Nedd8 activating enzyme hUba3 antibody
    • NEDD8-activating enzyme E1 catalytic subunit antibody
    • NEDD8-activating enzyme E1C antibody
    • uba3 antibody
    • UBA3 ubiquitin activating enzyme E1 homolog antibody
    • UBA3_HUMAN antibody
    • UBE1C antibody
    • Ubiquitin activating enzyme 3 antibody
    • Ubiquitin activating enzyme E1C antibody
    • Ubiquitin-activating enzyme 3 antibody
    • Ubiquitin-activating enzyme E1C antibody
    • Ubiquitin-like modifier-activating enzyme 3 antibody
    see all

Images

  • Anti-UBE1C antibody (ab38649) + mouse brain tissue lysate

    Predicted band size : 52 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,28 kDa,44 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab38649 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References

ab38649 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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