Application notesIs unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
FunctionCatalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
Tissue specificityUbiquitously expressed.
PathwayProtein modification; protein neddylation.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family. UBA3 subfamily.