SpecificityThe specificity of this antibody has been confirmed by immunoblot analyses of Ube1L-transfected CHO cells. CHO cells that did not basally express Ube1L mRNA were transfected with a full-length Ube1L cDNA or an insertless vector. CHO cells transfected with Ube1L expressed Ube1L, whereas cells transfected with an insertless vector did not.
This antibody reacts with the N-terminus of Ube1L.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Detects a band of approximately 112 kDa.
FunctionActivates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
Tissue specificityExpressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
Immunofluorescence was used to detect UBE1L protein expression in MDA-MB-231 cells (red staining) and DAPI staining revealed the nuclei (blue staining). The right panel shows immunofluorescence with no primary antibody.
This picture was kindly supplied as part of the review submitted by Sonia del Rincon.
References for Anti-Ube1L antibody (ab12199)
This product has been referenced in:
Chan EY et al. Quantitative analysis of human immunodeficiency virus type 1-infected CD4+ cell proteome: dysregulated cell cycle progression and nuclear transport coincide with robust virus production. J Virol81:7571-83 (2007).
Read more (PubMed: 17494070) »
Pitha-Rowe I et al. Involvement of UBE1L in ISG15 Conjugation during Retinoid-induced Differentiation of Acute Promyelocytic Leukemia. J Biol Chem279:18178-87 (2004).
Read more (PubMed: 14976209) »