Overview

  • Product nameAnti-Ube1L antibody
    See all Ube1L primary antibodies
  • Description
    Rabbit polyclonal to Ube1L
  • SpecificityThe specificity of this antibody has been confirmed by immunoblot analyses of Ube1L-transfected CHO cells. CHO cells that did not basally express Ube1L mRNA were transfected with a full-length Ube1L cDNA or an insertless vector. CHO cells transfected with Ube1L expressed Ube1L, whereas cells transfected with an insertless vector did not. This antibody reacts with the N-terminus of Ube1L.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide:

    DCDPRSIHVREDGSLEIGD

    , corresponding to amino acids 222-240 of Human Ube1L.

Properties

Applications

Our Abpromise guarantee covers the use of ab12199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000.
IHC-Fr 1/1000.
WB 1/500. Detects a band of approximately 112 kDa.

Target

  • FunctionActivates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
  • Tissue specificityExpressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • D8 antibody
    • MGC12713 antibody
    • UBA1, ubiquitin activating enzyme E1 homolog B antibody
    • UBA1B antibody
    • UBA7 antibody
    • UBA7, ubiquitin activating enzyme E1 antibody
    • UBA7_HUMAN antibody
    • Ube 1L antibody
    • UBE 2 antibody
    • UBE1L antibody
    • UBE2 antibody
    • Ubiquitin activating enzyme 2 antibody
    • Ubiquitin activating enzyme E1 homolog antibody
    • Ubiquitin Activating Enzyme E1 Like antibody
    • Ubiquitin activating enzyme E1 related protein antibody
    • Ubiquitin-activating enzyme 7 antibody
    • Ubiquitin-activating enzyme E1 homolog antibody
    • ubiquitin-activating enzyme-2 antibody
    • ubiquitin-like modifier activating enzyme 7 antibody
    • Ubiquitin-like modifier-activating enzyme 7 antibody
    see all

Anti-Ube1L antibody images

  • Immunohistochemistry using ab12200 (C-terminus) and ab12199 (N-terminus) with peptide blocking (right panel) and without peptide blocking (left panel).
  • Immunofluorescence was used to detect UBE1L protein expression in MDA-MB-231 cells (red staining) and DAPI staining revealed the nuclei (blue staining). The right panel shows immunofluorescence with no primary antibody.

    This picture was kindly supplied as part of the review submitted by Sonia del Rincon.

References for Anti-Ube1L antibody (ab12199)

This product has been referenced in:
  • Chan EY  et al. Quantitative analysis of human immunodeficiency virus type 1-infected CD4+ cell proteome: dysregulated cell cycle progression and nuclear transport coincide with robust virus production. J Virol 81:7571-83 (2007). WB ; Human . Read more (PubMed: 17494070) »
  • Pitha-Rowe I  et al. Involvement of UBE1L in ISG15 Conjugation during Retinoid-induced Differentiation of Acute Promyelocytic Leukemia. J Biol Chem 279:18178-87 (2004). Read more (PubMed: 14976209) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"