Overview

  • Product nameAnti-Ube1L antibody
    See all Ube1L primary antibodies
  • Description
    Mouse polyclonal to Ube1L
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human Ube1L (AAH06378)

  • Positive control
    • Human liver tissue lysate, Ube1L transfected 293T cell lysate, HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab89647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionActivates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
  • Tissue specificityExpressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • D8 antibody
    • MGC12713 antibody
    • UBA1, ubiquitin activating enzyme E1 homolog B antibody
    • UBA1B antibody
    • UBA7 antibody
    • UBA7, ubiquitin activating enzyme E1 antibody
    • UBA7_HUMAN antibody
    • Ube 1L antibody
    • UBE 2 antibody
    • UBE1L antibody
    • UBE2 antibody
    • Ubiquitin activating enzyme 2 antibody
    • Ubiquitin activating enzyme E1 homolog antibody
    • Ubiquitin Activating Enzyme E1 Like antibody
    • Ubiquitin activating enzyme E1 related protein antibody
    • Ubiquitin-activating enzyme 7 antibody
    • Ubiquitin-activating enzyme E1 homolog antibody
    • ubiquitin-activating enzyme-2 antibody
    • ubiquitin-like modifier activating enzyme 7 antibody
    • Ubiquitin-like modifier-activating enzyme 7 antibody
    see all

Anti-Ube1L antibody images

  • Anti-Ube1L antibody (ab89647) at 1 µg/ml + Human liver tissue lysate at 50 µg

    Predicted band size : 112 kDa
    Observed band size : 112 kDa
  • All lanes : Anti-Ube1L antibody (ab89647) at 1 µg/ml

    Lane 1 : Ube1L transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 112 kDa
  • ab89647 at 10µg/ml staining Ube1L in HeLa cells by Immunofluorescence.

References for Anti-Ube1L antibody (ab89647)

ab89647 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"