The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
1/10 - 1/100.
1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/100 - 1/250.
Application notesIs unsuitable for IHC-P.
FunctionActivates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
Tissue specificityExpressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family.