Overview

  • Product nameAnti-Ube2B antibody
    See all Ube2B primary antibodies
  • Description
    Rabbit polyclonal to Ube2B
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1 and 152 of Human Ube2B (NP_003328)

  • Positive control
    • HeLa and NIH-3T3 whole cell lysate. HepG2 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab96848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 17 kDa.

Target

  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Cellular localizationCell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2 17kDa antibody
    • E2 protein antibody
    • hHR6B antibody
    • HR6B antibody
    • RAD6 homolog B antibody
    • RAD6B antibody
    • UBC2 antibody
    • UBE2B antibody
    • UBE2B_HUMAN antibody
    • Ubiquitin carrier protein B antibody
    • Ubiquitin conjugating enzyme E2 B antibody
    • Ubiquitin conjugating enzyme E2B (RAD6 homolog) antibody
    • Ubiquitin conjugating enzyme E2B antibody
    • Ubiquitin protein ligase B antibody
    • Ubiquitin-conjugating enzyme E2 17 kDa antibody
    • Ubiquitin-conjugating enzyme E2 B antibody
    • Ubiquitin-conjugating enzyme E2-17 kDa antibody
    • Ubiquitin-protein ligase B antibody
    see all

Anti-Ube2B antibody images

  • Anti-Ube2B antibody (ab96848) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 17 kDa
  • Anti-Ube2B antibody (ab96848) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size : 17 kDa

References for Anti-Ube2B antibody (ab96848)

ab96848 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

Submitted Aug 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"