Anti-Ube2B antibody (ab96848)
Key features and details
- Rabbit polyclonal to Ube2B
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Ube2B antibody
See all Ube2B primary antibodies -
Description
Rabbit polyclonal to Ube2B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Zebrafish -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1 and 152 of Human Ube2B (NP_003328)
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Positive control
- HeLa and NIH-3T3 whole cell lysate. HepG2 cell line.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 17 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 17 kDa. |
Target
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Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. -
Cellular localization
Cell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei. - Information by UniProt
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Database links
- Entrez Gene: 7320 Human
- Entrez Gene: 22210 Mouse
- Entrez Gene: 81816 Rat
- Omim: 179095 Human
- SwissProt: P63146 Human
- SwissProt: P63147 Mouse
- SwissProt: P63149 Rat
- Unigene: 612096 Human
see all -
Alternative names
- E2 17kDa antibody
- E2 protein antibody
- hHR6B antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab96848 has not yet been referenced specifically in any publications.