Overview

  • Product nameAnti-UBE2C antibody
    See all UBE2C primary antibodies
  • Description
    Rabbit polyclonal to UBE2C
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (DTQGNICLDI LKEKWSALYD VRTILLSIQS LLGEPNIDSP LNTHAAELWK) of human UBE2C (NP_008950)

  • Positive control
    • Fetal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications
    Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cyclin selective ubiquitin carrier protein antibody
    • dJ447F3.2 antibody
    • Mitotic specific ubiquitin conjugating enzyme antibody
    • UB E2C antibody
    • UBCH 10 antibody
    • UbcH10 antibody
    • UBE 2C antibody
    • Ube2c antibody
    • UBE2C_HUMAN antibody
    • Ubiquitin carrier protein C antibody
    • Ubiquitin carrier protein E2 C antibody
    • Ubiquitin carrier protein E2C antibody
    • Ubiquitin conjugating enzyme E2 C antibody
    • Ubiquitin conjugating enzyme E2C antibody
    • Ubiquitin protein ligase C antibody
    • Ubiquitin-conjugating enzyme E2 C antibody
    • Ubiquitin-protein ligase C antibody
    see all

Anti-UBE2C antibody images

  • Anti-UBE2C antibody (ab80428) at 1 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 20 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

References for Anti-UBE2C antibody (ab80428)

ab80428 has not yet been referenced specifically in any publications.

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