Recombinant
RabMAb

Anti-UBE2C antibody [EPR5161(2)] (ab125002)

Overview

  • Product name
    Anti-UBE2C antibody [EPR5161(2)]
    See all UBE2C primary antibodies
  • Description
    Rabbit monoclonal [EPR5161(2)] to UBE2C
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human UBE2C.

  • Positive control
    • SW480, HeLa, MCF7 and 293T cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab125002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the ubiquitin-conjugating enzyme family.
    • Post-translational
      modifications
      Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cyclin selective ubiquitin carrier protein antibody
      • dJ447F3.2 antibody
      • Mitotic specific ubiquitin conjugating enzyme antibody
      • UB E2C antibody
      • UBCH 10 antibody
      • UbcH10 antibody
      • UBE 2C antibody
      • Ube2c antibody
      • UBE2C_HUMAN antibody
      • Ubiquitin carrier protein C antibody
      • Ubiquitin carrier protein E2 C antibody
      • Ubiquitin carrier protein E2C antibody
      • Ubiquitin conjugating enzyme E2 C antibody
      • Ubiquitin conjugating enzyme E2C antibody
      • Ubiquitin protein ligase C antibody
      • Ubiquitin-conjugating enzyme E2 C antibody
      • Ubiquitin-protein ligase C antibody
      see all

    Images

    • All lanes : Anti-UBE2C antibody [EPR5161(2)] (ab125002) at 1/1000 dilution

      Lane 1 : SW480 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 20 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab125002 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab125002.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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