Overview

  • Product nameAnti-UBE2CBP antibody
  • Description
    Rabbit polyclonal to UBE2CBP
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: SLVIESLRNS KYIKKFPLLE NTFKADSSSA WSAVKVLYQP CIKSRNEKLV SLWESDISVH PLTLPSATCL ELLL, corresponding to amino acids of Human UBE2CBP.

  • Positive control
    • Human heart muscle tissue; RT 4, U 251 MG, Human liver and tonsil lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab121927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome.
  • PathwayProtein modification; protein ubiquitination.
  • DomainThe C-terminal half (AA 188-389) is able to bind cyclin B and shows a self-ubiquitinylation activity (mono-, poly, or multi-ubiquitinylation) in a HECT-like sequence dependent manner.
  • Post-translational
    modifications
    Ubiquitinated by UBCH10 (E2 ubiquitin-conjugating enzyme).
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • C6orf157 antibody
    • chromosome 6 open reading frame 157 antibody
    • DKFZp434A1520 antibody
    • EC 6.3.2 antibody
    • EC 6.3.2.- antibody
    • H10BH antibody
    • MGC126561 antibody
    • MGC126587 antibody
    • OTTHUMP00000016789 antibody
    • OTTHUMP00000220801 antibody
    • UB2CB_HUMAN antibody
    • UBCH10 binding protein with a hect-like domain antibody
    • UbcH10-binding protein with a HECT-like domain antibody
    • UBE2CBP antibody
    • Ubiquitin-conjugating enzyme E2C-binding protein antibody
    • YJR141W antibody
    see all

Anti-UBE2CBP antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab121927 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-UBE2CBP antibody (ab121927) at 1/250 dilution

    Lane 1 : RT 4 cell line lysate
    Lane 2 : U 251 MG cell line lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate
  • ab121927, at 1/20, staining UBE2CBP in paraffin embedded Human heart muscle tissue by Immunohistochemistry.

References for Anti-UBE2CBP antibody (ab121927)

ab121927 has not yet been referenced specifically in any publications.

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