Overview

  • Product name
    Anti-UBE2CBP antibody
  • Description
    Rabbit polyclonal to UBE2CBP
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: SLVIESLRNS KYIKKFPLLE NTFKADSSSA WSAVKVLYQP CIKSRNEKLV SLWESDISVH PLTLPSATCL ELLL, corresponding to amino acids of Human UBE2CBP.

  • Positive control
    • Human heart muscle tissue; RT 4, U 251 MG, Human liver and tonsil lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab121927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome.
  • Pathway
    Protein modification; protein ubiquitination.
  • Domain
    The C-terminal half (AA 188-389) is able to bind cyclin B and shows a self-ubiquitinylation activity (mono-, poly, or multi-ubiquitinylation) in a HECT-like sequence dependent manner.
  • Post-translational
    modifications
    Ubiquitinated by UBCH10 (E2 ubiquitin-conjugating enzyme).
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • C6orf157 antibody
    • chromosome 6 open reading frame 157 antibody
    • DKFZp434A1520 antibody
    • EC 6.3.2 antibody
    • EC 6.3.2.- antibody
    • H10BH antibody
    • MGC126561 antibody
    • MGC126587 antibody
    • OTTHUMP00000016789 antibody
    • OTTHUMP00000220801 antibody
    • UB2CB_HUMAN antibody
    • UBCH10 binding protein with a hect-like domain antibody
    • UbcH10-binding protein with a HECT-like domain antibody
    • UBE2CBP antibody
    • Ubiquitin-conjugating enzyme E2C-binding protein antibody
    • YJR141W antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab121927 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-UBE2CBP antibody (ab121927) at 1/250 dilution

    Lane 1 : RT 4 cell line lysate
    Lane 2 : U 251 MG cell line lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate
  • ab121927, at 1/20, staining UBE2CBP in paraffin embedded Human heart muscle tissue by Immunohistochemistry.

References

ab121927 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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