Overview

  • Product nameAnti-UBE2D1 antibody
    See all UBE2D1 primary antibodies
  • Description
    Rabbit polyclonal to UBE2D1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Cow, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide: SKVLLSICSL LCDPNPDDPL VPDIAQIYKS DKEKYNRHAR EWTQKYAM, corresponding to amino acids 100-149 of Human UBE2D1

  • Positive control
    • Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab66600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:62500.

Target

  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of CHIP, TRAF6 and TRIM63/MURF1. Ubiquitinates CHIP-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.
  • Tissue specificityUbiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2(17)KB1 antibody
    • SFT antibody
    • Stimulator of Fe transport antibody
    • UB2D1_HUMAN antibody
    • UBC 4/5 antibody
    • UBC4/5 antibody
    • UBC4/5 homolog antibody
    • UBC4/5, S. cerevisiae, homolog of antibody
    • UBCH 5 antibody
    • UBCH 5A antibody
    • UbcH5 antibody
    • UBCH5A antibody
    • Ube2d1 antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin carrier protein D1 antibody
    • Ubiquitin conjugating enzyme E2 17 kDa 1 antibody
    • Ubiquitin conjugating enzyme E2 D1 antibody
    • Ubiquitin conjugating enzyme E2D 1 (UBC4/5 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2D 1 antibody
    • Ubiquitin conjugating enzyme UBCH5A antibody
    • Ubiquitin protein ligase D1 antibody
    • Ubiquitin-conjugating enzyme E2 D1 antibody
    • Ubiquitin-conjugating enzyme E2(17)KB 1 antibody
    • Ubiquitin-conjugating enzyme E2-17 kDa 1 antibody
    • Ubiquitin-protein ligase D1 antibody
    see all

Anti-UBE2D1 antibody images

  • All lanes : Anti-UBE2D1 antibody (ab66600) at 1.25 µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : Molecular marker
    Lane 2 : jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

References for Anti-UBE2D1 antibody (ab66600)

ab66600 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"