Anti-UBE2D2 antibody (ab66602)

Overview

  • Product nameAnti-UBE2D2 antibody
    See all UBE2D2 primary antibodies
  • Description
    Rabbit polyclonal to UBE2D2
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Pig, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: ISKVLLSICS LLCDPNPDDP LVPEIARIYK TDREKYNRIA REWTQKYAM, corresponding to C terminal amino acids 99-147 of Human UBE2D2

  • Positive control
    • Jurkat cells. Lung.

Properties

Applications

Our Abpromise guarantee covers the use of ab66602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2(17)KB2 antibody
    • OTTHUMP00000223475 antibody
    • OTTHUMP00000223476 antibody
    • OTTHUMP00000223477 antibody
    • OTTHUMP00000223478 antibody
    • OTTHUMP00000224375 antibody
    • PUBC 1 antibody
    • PUBC1 antibody
    • UB2D2_HUMAN antibody
    • UBC 4 antibody
    • UBC 4/5 antibody
    • UBC4 antibody
    • UBC4/5 antibody
    • UBC4/5 homolog yeast antibody
    • UBCH 5B antibody
    • UBCH5B antibody
    • UBE2D2 antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin carrier protein D2 antibody
    • Ubiquitin conjugating enzyme E2 17 kDa 2 antibody
    • Ubiquitin conjugating enzyme E2 D2 antibody
    • Ubiquitin conjugating enzyme E2D 2 (homologous to yeast UBC4/5) antibody
    • Ubiquitin conjugating enzyme E2D 2 (UBC4/5 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2D 2 antibody
    • Ubiquitin protein ligase D2 antibody
    • Ubiquitin-conjugating enzyme E2 D2 antibody
    • Ubiquitin-conjugating enzyme E2(17)KB 2 antibody
    • Ubiquitin-conjugating enzyme E2-17 kDa 2 antibody
    • Ubiquitin-protein ligase D2 antibody
    see all

Anti-UBE2D2 antibody images

  • Anti-UBE2D2 antibody (ab66602) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
    Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 15%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling UBE2D2 with ab66602 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.



References for Anti-UBE2D2 antibody (ab66602)

ab66602 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The C. elegans ortholog is called LEThal family member (let-70). Here is a link to the NCBI record for the protein: http://www.ncbi.nlm.nih.gov/protein/P35129.1 . I hope this information is helpful to ...

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Thank you for contacting us. My BLAST search of the amino acid range listed in the immunogen field on the datasheet just turned up a gene from C. vulgaris, Cre-Let-70. I am going to ask the laboratory that produces the antibody to help with this one an...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
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Dr. Kirk McManus

Verified customer

Submitted Jun 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"