Overview

  • Product nameAnti-UBE2D3 antibody
    See all UBE2D3 primary antibodies
  • Description
    Rabbit polyclonal to UBE2D3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Pig, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human UBE2D3.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; MCF7; Jurkat; NIH3T3; MDA MB 231; MDA MB 361.

Properties

Applications

Our Abpromise guarantee covers the use of ab106315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

Target

  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. Supports NRDP1-mediated ubiquitination and degradation of ERBB3 and of BRUCE which triggers apoptosis. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Cellular localizationCell membrane. Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2(17)KB3 antibody
    • MGC43926 antibody
    • MGC5416 antibody
    • PRO2116 antibody
    • UB2D3_HUMAN antibody
    • UBC 4/5 antibody
    • UBC4/5 antibody
    • UBC4/5 homolog yeast antibody
    • UBC4/5, S. cerevisiae, homolog of antibody
    • UBCH 5C antibody
    • UBCH5C antibody
    • Ube2d3 antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin carrier protein D3 antibody
    • Ubiquitin conjugating enzyme E2 17 kDa 3 antibody
    • Ubiquitin conjugating enzyme E2 D3 antibody
    • Ubiquitin conjugating enzyme E2D 3 (homologous to yeast UBC4/5) antibody
    • Ubiquitin conjugating enzyme E2D 3 (UBC4/5 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2D 3 antibody
    • Ubiquitin protein ligase D3 antibody
    • Ubiquitin-conjugating enzyme E2 D3 antibody
    • Ubiquitin-conjugating enzyme E2(17)KB 3 antibody
    • Ubiquitin-conjugating enzyme E2-17 kDa 3 antibody
    • Ubiquitin-protein ligase D3 antibody
    see all

Anti-UBE2D3 antibody images

  • All lanes : Anti-UBE2D3 antibody (ab106315) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 6 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 17 kDa


    Exposure time : 20 minutes

References for Anti-UBE2D3 antibody (ab106315)

This product has been referenced in:
  • Fan Y  et al. USP21 negatively regulates antiviral response by acting as a RIG-I deubiquitinase. J Exp Med 211:313-28 (2014). Mouse . Read more (PubMed: 24493797) »

See 1 Publication for this product

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