• Product name
  • Description
    Mouse monoclonal to UBE2D3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-148 of Human UBE2D3

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.



Our Abpromise guarantee covers the use of ab58251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 17 kDa.


  • Function
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. Supports NRDP1-mediated ubiquitination and degradation of ERBB3 and of BRUCE which triggers apoptosis. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family.
  • Cellular localization
    Cell membrane. Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2(17)KB3 antibody
    • MGC43926 antibody
    • MGC5416 antibody
    • PRO2116 antibody
    • UB2D3_HUMAN antibody
    • UBC 4/5 antibody
    • UBC4/5 antibody
    • UBC4/5 homolog yeast antibody
    • UBC4/5, S. cerevisiae, homolog of antibody
    • UBCH 5C antibody
    • UBCH5C antibody
    • Ube2d3 antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin carrier protein D3 antibody
    • Ubiquitin conjugating enzyme E2 17 kDa 3 antibody
    • Ubiquitin conjugating enzyme E2 D3 antibody
    • Ubiquitin conjugating enzyme E2D 3 (homologous to yeast UBC4/5) antibody
    • Ubiquitin conjugating enzyme E2D 3 (UBC4/5 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2D 3 antibody
    • Ubiquitin protein ligase D3 antibody
    • Ubiquitin-conjugating enzyme E2 D3 antibody
    • Ubiquitin-conjugating enzyme E2(17)KB 3 antibody
    • Ubiquitin-conjugating enzyme E2-17 kDa 3 antibody
    • Ubiquitin-protein ligase D3 antibody
    see all

Anti-UBE2D3 antibody images

  • Predicted band size : 17 kDa
    UBE2D3 antibody (ab58251) at 1ug/lane + Jurkat cell lysate at 25ug/lane.

References for Anti-UBE2D3 antibody (ab58251)

This product has been referenced in:
  • Uhler JP  et al. The UbL protein UBTD1 stably interacts with the UBE2D family of E2 ubiquitin conjugating enzymes. Biochem Biophys Res Commun 443:7-12 (2014). WB . Read more (PubMed: 24211586) »
  • Ranaweera RS & Yang X Auto-ubiquitination of Mdm2 enhances its substrate ubiquitin ligase activity. J Biol Chem 288:18939-46 (2013). WB ; Human . Read more (PubMed: 23671280) »

See all 2 Publications for this product

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