The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: 1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 12 kDa). [A fusion protein was used to produce this Western Blot, hence the difference in M.Wt compared to the native protein.]
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.