General notesab94306 is a 293T cell transfected lysate in which Human Ube2G1 has been transiently over-expressed using a pCMV-Ube2G1 plasmid. The lysate is provided in 1X Sample Buffer.
Note: For more details about how the transfected lysate was prepared view preparation notes
BackgroundFunction: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4.
Tissue specificity: Widely expressed, mainly in skeletal muscle.
Pathway: Protein modification; protein ubiquitination.
Similarity: Belongs to the ubiquitin-conjugating enzyme family.