Ube2G1 overexpression 293T lysate (whole cell) (ab94306)

Overview

  • Product nameUbe2G1 overexpression 293T lysate (whole cell)
  • Description
    Ube2G1 overexpression lysate
  • General notesab94306 is a 293T cell transfected lysate in which Human Ube2G1 has been transiently over-expressed using a pCMV-Ube2G1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4. Tissue specificity: Widely expressed, mainly in skeletal muscle. Pathway: Protein modification; protein ubiquitination. Similarity: Belongs to the ubiquitin-conjugating enzyme family.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Ube2G1 overexpression 293T lysate (whole cell) images

  • ab94306 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Ube2G1 antibody (ab58256) at 1/500 dilution

    Lane 1 : Ube2G1 overexpression 293T lysate (whole cell) (ab94306)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

References for Ube2G1 overexpression 293T lysate (whole cell) (ab94306)

ab94306 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"