Overview

  • Product nameAnti-UBE2I / UBC9 antibody
    See all UBE2I / UBC9 primary antibodies
  • Description
    Goat polyclonal to UBE2I / UBC9
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Hamster, Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    SGIALSRLAQERK

    , corresponding to N terminal amino acids 2-14 of Human Ube2I.

  • Positive control
    • Human Kidney, Human Lung and Human Testis lysates
  • General notesProduct is very sensitive to freeze/thawing therefore this should be avoided. Store at 4C for short term use and -20C for long term storage in small aliquots.

Properties

Applications

Our Abpromise guarantee covers the use of ab21193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. See Abreview.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa). Transfer onto PVDF membrane and block in 3 % milk. Incubate primary antibody with membrane at room temperature.
ELISA 1/64000.

Target

  • FunctionAccepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.
  • Tissue specificityExpressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
  • PathwayProtein modification; protein sumoylation.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Cellular localizationNucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body.
  • Information by UniProt
  • Database links
  • Alternative names
    • C358B7.1 antibody
    • p18 antibody
    • SUMO 1 protein ligase antibody
    • SUMO conjugating enzyme UBC9 antibody
    • SUMO-conjugating enzyme UBC9 antibody
    • SUMO-protein ligase antibody
    • SUMO1 protein ligase antibody
    • UBC9 antibody
    • UBC9_HUMAN antibody
    • UBCE9 antibody
    • Ube2i antibody
    • Ubiquitin carrier protein 9 antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin carrier protein I antibody
    • Ubiquitin conjugating enzyme 9 antibody
    • Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) antibody
    • Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast) antibody
    • Ubiquitin conjugating enzyme UbcE2A antibody
    • Ubiquitin like protein SUMO 1 conjugating enzyme antibody
    • Ubiquitin protein ligase E2I antibody
    • Ubiquitin-conjugating enzyme E2 I antibody
    • Ubiquitin-protein ligase I antibody
    see all

Anti-UBE2I / UBC9 antibody images

  • Anti-UBE2I / UBC9 antibody (ab21193) at 0.1 µg/ml + Human kidney lysate at 35 µg

    Predicted band size : 18 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Ab21193 (0.3ug/ml) staining human UBE2I in human kidney by immunohistochemistry using paraffin embedded tissue.

    Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

  • ab21193 staining UBE2I / UBC9 in mouse oocyte by Immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% Tween and blocked in 0.1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/50 and incubated with sample for 1 hour at 25°C. A Cy5® conjugated Donkey polyclonal to goat, diluted 1/400 was used as the secondary.

    See Abreview

References for Anti-UBE2I / UBC9 antibody (ab21193)

This product has been referenced in:
  • Gross TJ  et al. A microRNA processing defect in smokers' macrophages is linked to SUMOylation of the endonuclease DICER. J Biol Chem 289:12823-34 (2014). WB ; Human . Read more (PubMed: 24668803) »
  • Williams MJ  et al. Obesity-linked homologues TfAP-2 and Twz establish meal frequency in Drosophila melanogaster. PLoS Genet 10:e1004499 (2014). Read more (PubMed: 25187989) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human fibroblast)
Loading amount 10 µg
Specification Human fibroblast
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Nupage)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (oocyte, embryo)
Specification oocyte, embryo
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 25°C
Username

Dr. Motomasa Ihara

Verified customer

Submitted Nov 04 2008

Thank you for your enquiry. All of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as we receive additional data. The information you have requested for can be f...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"