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Our Abpromise guarantee covers the use of ab21193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution. See Abreview.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa). Transfer onto PVDF membrane and block in 3 % milk. Incubate primary antibody with membrane at room temperature.|
Primary incubation was 1 hour. Detected by chemiluminescence.
Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
ab21193 staining UBE2I / UBC9 in mouse oocyte by Immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% Tween and blocked in 0.1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/50 and incubated with sample for 1 hour at 25°C. A Cy5® conjugated Donkey polyclonal to goat, diluted 1/400 was used as the secondary.