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Synthetic peptide: AVPNLAGLQQ ANRHHGLLGG ALANLFVIVG FAAFAYTVKY VLRSIAQE, corresponding to amino acids 211-259 of Human UBC6
Our Abpromise guarantee covers the use of ab59012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay 1/312500.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling UBE2J2 with ab59012 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of villus. Magnification: 400X.
ab59012 has not yet been referenced specifically in any publications.