Overview

  • Product nameAnti-UBE2J2 antibodySee all UBE2J2 primary antibodies ...
  • Description
    Rabbit polyclonal to UBE2J2
  • Tested applicationsELISA, WB, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: AVPNLAGLQQ ANRHHGLLGG ALANLFVIVG FAAFAYTVKY VLRSIAQE, corresponding to amino acids 211-259 of Human UBC6

  • Positive control
    • HepG2 cell lysate; Human intestine tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab59012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Anti-UBE2J2 antibody images

  • Anti-UBE2J2 antibody (ab59012) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
    Diluted in 5% skim milk/PBS buffer; gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling UBE2J2 with ab59012 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of villus. Magnification: 400X.

References for Anti-UBE2J2 antibody (ab59012)

ab59012 has not yet been referenced specifically in any publications.

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