Overview

  • Product nameAnti-Ube2L3 antibody
    See all Ube2L3 primary antibodies
  • Description
    Rabbit polyclonal to Ube2L3
  • Tested applicationsWB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide: SLIALVNDPQ PEHPLRADLA EEYSKDRKKF CKNAEEFTKK YGEKRPVD, corresponding to C terminal amino acids 106-155 of Human Ube2L3

  • Positive control
    • Jurkat cell lysate Human intestine tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab66944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
  • Tissue specificityUbiquitous, with highest expression in testis.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications
    Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2 F1 antibody
    • E2-F1 antibody
    • L UBC antibody
    • L-UBC antibody
    • UB2L3_HUMAN antibody
    • UBCE7 antibody
    • UbcH7 antibody
    • UbcM4 antibody
    • Ube2l3 antibody
    • Ubiquitin carrier protein L3 antibody
    • Ubiquitin conjugating enzyme E2 L3 antibody
    • Ubiquitin protein ligase L3 antibody
    • Ubiquitin-conjugating enzyme E2 L3 antibody
    • Ubiquitin-conjugating enzyme E2-F1 antibody
    • Ubiquitin-protein ligase L3 antibody
    see all

Anti-Ube2L3 antibody images

  • Anti-Ube2L3 antibody (ab66944) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 18 kDa
    Observed band size : 18 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analyisi of human intestine tissue labelling Ube2L3 with ab66944 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the intestinal villus. Magnification: 400X.

References for Anti-Ube2L3 antibody (ab66944)

ab66944 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"