Overview

  • Product name
  • Description
    Rabbit polyclonal to Ube2L3
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region between residue 100 and the C-terminus (residue 154) of human Ube2L3 (NP_003338.1).

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab72545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IP Use at 2-5 µg/mg of lysate.

Efficiency in IP is low.

Target

  • Function
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
  • Tissue specificity
    Ubiquitous, with highest expression in testis.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications
    Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2 F1 antibody
    • L UBC antibody
    • L-UBC antibody
    • UB2L3_HUMAN antibody
    • UBCE7 antibody
    • UbcH7 antibody
    • UbcM4 antibody
    • Ube2l3 antibody
    • Ubiquitin carrier protein L3 antibody
    • Ubiquitin conjugating enzyme E2 L3 antibody
    • Ubiquitin protein ligase L3 antibody
    • Ubiquitin-conjugating enzyme E2 L3 antibody
    • Ubiquitin-conjugating enzyme E2-F1 antibody
    • Ubiquitin-protein ligase L3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Ube2L3 with ab72545 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-Ube2L3 antibody (ab72545) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 18 kDa
    Observed band size : 18 kDa
  • Detection of Human Ube2L3 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72545 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

References

ab72545 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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