Overview

  • Product nameAnti-Ube2L3 antibody
    See all Ube2L3 primary antibodies
  • Description
    Rabbit polyclonal to Ube2L3
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 36-85 (QGLIVPDNPP YDKGAFRIEI NFPAEYPFKP PKITFKTKIY HPNIDEKGQV) of Human Ube2L3 (NP_003338).

  • Positive control
    • Ube2L3 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab89065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
  • Tissue specificityUbiquitous, with highest expression in testis.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications
    Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2 F1 antibody
    • L UBC antibody
    • L-UBC antibody
    • UB2L3_HUMAN antibody
    • UBCE7 antibody
    • UbcH7 antibody
    • UbcM4 antibody
    • Ube2l3 antibody
    • Ubiquitin carrier protein L3 antibody
    • Ubiquitin conjugating enzyme E2 L3 antibody
    • Ubiquitin protein ligase L3 antibody
    • Ubiquitin-conjugating enzyme E2 L3 antibody
    • Ubiquitin-conjugating enzyme E2-F1 antibody
    • Ubiquitin-protein ligase L3 antibody
    see all

Anti-Ube2L3 antibody images

  • Anti-Ube2L3 antibody (ab89065) at 1 µg/ml + Ube2L3 transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 18 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 10-20%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Ube2L3 with ab89065 at 4-8µg/ml.

References for Anti-Ube2L3 antibody (ab89065)

ab89065 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"