Overview

  • Product nameAnti-Ube2L3 antibody
    See all Ube2L3 primary antibodies
  • Description
    Rabbit polyclonal to Ube2L3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 92-154 of Human Ube2L3 (NP_003338).

  • Positive control
    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lysate; ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab97277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
  • Tissue specificityUbiquitous, with highest expression in testis.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications
    Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2 F1 antibody
    • L UBC antibody
    • L-UBC antibody
    • UB2L3_HUMAN antibody
    • UBCE7 antibody
    • UbcH7 antibody
    • UbcM4 antibody
    • Ube2l3 antibody
    • Ubiquitin carrier protein L3 antibody
    • Ubiquitin conjugating enzyme E2 L3 antibody
    • Ubiquitin protein ligase L3 antibody
    • Ubiquitin-conjugating enzyme E2 L3 antibody
    • Ubiquitin-conjugating enzyme E2-F1 antibody
    • Ubiquitin-protein ligase L3 antibody
    see all

Anti-Ube2L3 antibody images

  • All lanes : Anti-Ube2L3 antibody (ab97277) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 18 kDa
  • ab97277 at 1/200 dilution staining Ube2L3 in paraformaldehyde-fixed HeLa cells by immunofluorescence. The lower image shows ab97277 merged with a DNA probe.

References for Anti-Ube2L3 antibody (ab97277)

ab97277 has not yet been referenced specifically in any publications.

Product Wall

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