Overview

  • Product nameAnti-Ube2L3 antibody [EPR4368]
    See all Ube2L3 primary antibodies
  • Description
    Rabbit monoclonal [EPR4368] to Ube2L3
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Ube2L3

  • Positive control
    • WB: HeLa, LNCaP, Jurkat and K562 cell lysates IHC-P: Human testis tissue IF: HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab108936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
    • Tissue specificityUbiquitous, with highest expression in testis.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
    • Post-translational
      modifications
      Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation.
    • Cellular localizationNucleus. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • E2 F1 antibody
      • L UBC antibody
      • L-UBC antibody
      • UB2L3_HUMAN antibody
      • UBCE7 antibody
      • UbcH7 antibody
      • UbcM4 antibody
      • Ube2l3 antibody
      • Ubiquitin carrier protein L3 antibody
      • Ubiquitin conjugating enzyme E2 L3 antibody
      • Ubiquitin protein ligase L3 antibody
      • Ubiquitin-conjugating enzyme E2 L3 antibody
      • Ubiquitin-conjugating enzyme E2-F1 antibody
      • Ubiquitin-protein ligase L3 antibody
      see all

    Anti-Ube2L3 antibody [EPR4368] images

    • All lanes : Anti-Ube2L3 antibody [EPR4368] (ab108936) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : LNCaP cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 18 kDa
    • ab108936, at a 1/100 dilution, staining Ube2L3 in paraffin-embedded Human testis tissue
    • ab108936, at a 1/100 dilution, staining Ube2L3 in HeLa cells

    References for Anti-Ube2L3 antibody [EPR4368] (ab108936)

    ab108936 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"