Overview

  • Product nameAnti-Ube2L6 antibody [K1H3]
    See all Ube2L6 primary antibodies
  • Description
    Mouse monoclonal [K1H3] to Ube2L6
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-152 of Human Ube2L6 purified from E. coli (NP_004214).

  • Positive control
    • Human spleen, thymus and tonsil tissues; MCF7 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab115524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Predicted molecular weight: 18 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.

Target

  • FunctionCatalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53.
  • Tissue specificityPresent in natural killer cells (at protein level).
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications
    ISGylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC40331 antibody
    • Retinoic acid induced gene B protein antibody
    • Retinoic acid-induced gene B protein antibody
    • RIG B antibody
    • RIG-B antibody
    • UB2L6_HUMAN antibody
    • UBCH 8 antibody
    • UbcH8 antibody
    • UBE2L6 antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin carrier protein L6 antibody
    • Ubiquitin conjugating enzyme E2L 6 antibody
    • Ubiquitin protein ligase antibody
    • Ubiquitin protein ligase L6 antibody
    • Ubiquitin-protein ligase L6 antibody
    • Ubiquitin/ISG15 conjugating enzyme E2 L6 antibody
    • Ubiquitin/ISG15-conjugating enzyme E2 L6 antibody
    see all

Anti-Ube2L6 antibody [K1H3] images

  • Anti-Ube2L6 antibody [K1H3] (ab115524) at 1/2000 dilution + MCF7 cell lysate at 20 µg
    Developed using the ECL technique

    Predicted band size : 18 kDa
  • ab115524 at 5ug/ml staining Ube2L6 in Formalin-fixed, Paraffin-embedded Human Spleen tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115524 at 5ug/ml staining Ube2L6 in Formalin-fixed, Paraffin-embedded Human Thymus tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115524 at 5ug/ml staining Ube2L6 in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-Ube2L6 antibody [K1H3] (ab115524)

ab115524 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"