Ube2L6 overexpression 293T lysate (whole cell) (ab94307)

Overview

  • Product nameUbe2L6 overexpression 293T lysate (whole cell)
  • Description
    Ube2L6 overexpression lysate
  • General notesab94307 is a 293T cell transfected lysate in which Human Ube2L6 has been transiently over-expressed using a pCMV-Ube2L6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Tissue specificity: Present in natural killer cells (at protein level). Pathway: Protein modification; protein ubiquitination. Similarity: Belongs to the ubiquitin-conjugating enzyme family. PTM: ISGylated.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Ube2L6 overexpression 293T lysate (whole cell) images

  • ab94307 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Ube2L6 antibody (ab56502) at 1/500 dilution

    Lane 1 : Ube2L6 293T Transfected Lysate - Positive Control (ab94307)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

References for Ube2L6 overexpression 293T lysate (whole cell) (ab94307)

ab94307 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"