Overview

  • Product nameAnti-UBE2M antibody
    See all UBE2M primary antibodies
  • Description
    Rabbit polyclonal to UBE2M
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    RGGYIGSTYFER

    , corresponding to amino acids 169-180 of Human UBE2M, conjugated to KLH.

  • Positive control
    • IHC-P: Human kidney tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab116248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 21 kDa.
ELISA 1/2000 - 1/10000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionAccepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
  • PathwayProtein modification; protein neddylation.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
  • DomainBoth the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
  • Information by UniProt
  • Database links
  • Alternative names
    • hUbc12 antibody
    • NEDD8 carrier protein antibody
    • NEDD8 conjugating enzyme Ubc12 antibody
    • NEDD8 protein ligase antibody
    • NEDD8-conjugating enzyme Ubc12 antibody
    • UBC-RS2 antibody
    • UBC12 antibody
    • UBC12_HUMAN antibody
    • ube2m antibody
    • ubiquitin carrier protein M antibody
    • Ubiquitin conjugating enzyme E2 M antibody
    • Ubiquitin-conjugating enzyme E2 M antibody
    • ubiquitin-protein ligase M antibody
    • yeast UBC12 homolog antibody
    see all

Anti-UBE2M antibody images

  • ab116248, at 2.5 µg/ml, staining UBE2M in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References for Anti-UBE2M antibody (ab116248)

ab116248 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab116248.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"