Overview

  • Product name
  • Description
    Rabbit polyclonal to UBE2M
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Monkey, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-147 of Human UBE2M (Uniprot ID P51081).

  • Positive control
    • 293T whole cell lysate; Human Gastric cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
  • Pathway
    Protein modification; protein neddylation.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
  • Domain
    Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
  • Information by UniProt
  • Database links
  • Alternative names
    • hUbc12 antibody
    • NEDD8 carrier protein antibody
    • NEDD8 conjugating enzyme Ubc12 antibody
    • NEDD8 protein ligase antibody
    • NEDD8-conjugating enzyme Ubc12 antibody
    • UBC-RS2 antibody
    • UBC12 antibody
    • UBC12_HUMAN antibody
    • ube2m antibody
    • ubiquitin carrier protein M antibody
    • Ubiquitin conjugating enzyme E2 M antibody
    • Ubiquitin-conjugating enzyme E2 M antibody
    • ubiquitin-protein ligase M antibody
    • yeast UBC12 homolog antibody
    see all

Images

  • Anti-UBE2M antibody (ab155783) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 21 kDa
  • Immunohistochemical analysis of paraffin-embedded Human Gastric cancer tissue, labeling UBE2M with ab155783 at 1/100 dilution.

References

ab155783 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155783.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up