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Overview

  • Product name
    Anti-UBE2M antibody [EPR5333]
    See all UBE2M primary antibodies
  • Description
    Rabbit monoclonal [EPR5333] to UBE2M
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C-terminus in Human UBE2M.

  • Positive control
    • 293T and Ramos cell lysates, Human colonic adenocarcinoma and Human lung carcinoma tissues
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
  • Pathway
    Protein modification; protein neddylation.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
  • Domain
    Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
  • Information by UniProt
  • Database links
    • Alternative names
      • hUbc12 antibody
      • NEDD8 carrier protein antibody
      • NEDD8 conjugating enzyme Ubc12 antibody
      • NEDD8 protein ligase antibody
      • NEDD8-conjugating enzyme Ubc12 antibody
      • UBC-RS2 antibody
      • UBC12 antibody
      • UBC12_HUMAN antibody
      • ube2m antibody
      • ubiquitin carrier protein M antibody
      • Ubiquitin conjugating enzyme E2 M antibody
      • Ubiquitin-conjugating enzyme E2 M antibody
      • ubiquitin-protein ligase M antibody
      • yeast UBC12 homolog antibody
      see all

    Anti-UBE2M antibody [EPR5333] images

    • Flow Cytometry - Anti-UBE2M antibody [EPR5333] (ab109507)
      Flow Cytometry - Anti-UBE2M antibody [EPR5333] (ab109507)
      Overlay histogram showing Ramos cells stained with ab109507 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109507, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Western blot - UBE2M antibody [EPR5333] (ab109507)
      Western blot - UBE2M antibody [EPR5333] (ab109507)
      All lanes : Anti-UBE2M antibody [EPR5333] (ab109507) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 21 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2M antibody [EPR5333] (ab109507)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2M antibody [EPR5333] (ab109507)
      Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue using ab109507 at a dilution of 1/100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2M antibody [EPR5333] (ab109507)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2M antibody [EPR5333] (ab109507)
      Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue using ab109507 at a dilution of 1/100.

    References for Anti-UBE2M antibody [EPR5333] (ab109507)

    This product has been referenced in:
    • Coleman KE  et al. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. Elife 6:N/A (2017). Read more (PubMed: 28475037) »
    • Xu J  et al. The neddylation-cullin 2-RBX1 E3 ligase axis targets tumor suppressor RhoB for degradation in liver cancer. Mol Cell Proteomics 14:499-509 (2015). Read more (PubMed: 25540389) »

    See all 2 Publications for this product

    Publishing research using ab109507? Please let us know so that we can cite the reference in this datasheet.

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