Recombinant
Anti-UBE2M antibody [EPR5333] (ab109507)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-UBE2M antibody [EPR5333]
See all UBE2M primary antibodies -
DescriptionRabbit monoclonal [EPR5333] to UBE2M
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human UBE2M aa 150-250 (C terminal). The exact sequence is proprietary.
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Positive control
- 293T and Ramos cell lysates, Human colonic adenocarcinoma and Human lung carcinoma tissues
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR5333
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab109507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa). | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC | 1/500 - 1/1000. | |
| Flow Cyt | 1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionAccepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
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PathwayProtein modification; protein neddylation.
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Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
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DomainBoth the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
- Information by UniProt
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Database links
- Entrez Gene: 9040 Human
- Entrez Gene: 22192 Mouse
- Entrez Gene: 361509 Rat
- Omim: 603173 Human
- SwissProt: P61081 Human
- SwissProt: P61082 Mouse
- Unigene: 406068 Human
- Unigene: 196580 Mouse
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Alternative names
- hUbc12 antibody
- NEDD8 carrier protein antibody
- NEDD8 conjugating enzyme Ubc12 antibody
see all
Images
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![Flow Cytometry - Anti-UBE2M antibody [EPR5333] (ab109507)](http://a.abcam.com/ps/products/109/ab109507/Images/ab109507-4-ab109507FC.jpg)
Flow Cytometry - Anti-UBE2M antibody [EPR5333] (ab109507)Overlay histogram showing Ramos cells stained with ab109507 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109507, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Western blot - Anti-UBE2M antibody [EPR5333] (ab109507)](http://a.abcam.com/ps/products/109/ab109507/Images/UBE2M-Primary-antibodies-ab109507-2.jpg)
Western blot - Anti-UBE2M antibody [EPR5333] (ab109507)All lanes : Anti-UBE2M antibody [EPR5333] (ab109507) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 21 kDa
Observed band size: 20 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2M antibody [EPR5333] (ab109507)](http://a.abcam.com/ps/products/109/ab109507/Images/UBE2M-Primary-antibodies-ab109507-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2M antibody [EPR5333] (ab109507)Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using ab109507 at a dilution of 1/100.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2M antibody [EPR5333] (ab109507)](http://a.abcam.com/ps/products/109/ab109507/Images/UBE2M-Primary-antibodies-ab109507-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2M antibody [EPR5333] (ab109507)Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue using ab109507 at a dilution of 1/100.
Protocols
References
This product has been referenced in:
- Coleman KE et al. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. Elife 6:N/A (2017). Read more (PubMed: 28475037) »
- Xu J et al. The neddylation-cullin 2-RBX1 E3 ligase axis targets tumor suppressor RhoB for degradation in liver cancer. Mol Cell Proteomics 14:499-509 (2015). Read more (PubMed: 25540389) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"