• Product nameAnti-UBE2M antibody [EPR5333]
    See all UBE2M primary antibodies
  • Description
    Rabbit monoclonal [EPR5333] to UBE2M
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C-terminus in Human UBE2M.

  • Positive control
    • 293T and Ramos cell lysates, Human colonic adenocarcinoma and Human lung carcinoma tissues
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab109507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • FunctionAccepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
  • PathwayProtein modification; protein neddylation.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
  • DomainBoth the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
  • Information by UniProt
  • Database links
  • Alternative names
    • hUbc12 antibody
    • NEDD8 carrier protein antibody
    • NEDD8 conjugating enzyme Ubc12 antibody
    • NEDD8 protein ligase antibody
    • NEDD8-conjugating enzyme Ubc12 antibody
    • UBC-RS2 antibody
    • UBC12 antibody
    • UBC12_HUMAN antibody
    • ube2m antibody
    • ubiquitin carrier protein M antibody
    • Ubiquitin conjugating enzyme E2 M antibody
    • Ubiquitin-conjugating enzyme E2 M antibody
    • ubiquitin-protein ligase M antibody
    • yeast UBC12 homolog antibody
    see all

Anti-UBE2M antibody [EPR5333] images

  • Overlay histogram showing Ramos cells stained with ab109507 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109507, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-UBE2M antibody [EPR5333] (ab109507) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : Ramos cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size : 21 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue using ab109507 at a dilution of 1/100.
  • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue using ab109507 at a dilution of 1/100.

References for Anti-UBE2M antibody [EPR5333] (ab109507)

ab109507 has not yet been referenced specifically in any publications.

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