Overview

  • Product nameAnti-UBE2M antibody [EPR5334]
    See all UBE2M primary antibodies
  • Description
    Rabbit monoclonal [EPR5334] to UBE2M
  • Tested applicationsSuitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human UBE2M

  • Positive control
    • Human 293T and Ramos cell line lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • FunctionAccepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
    • PathwayProtein modification; protein neddylation.
    • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
    • DomainBoth the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
    • Information by UniProt
    • Database links
    • Alternative names
      • hUbc12 antibody
      • NEDD8 carrier protein antibody
      • NEDD8 conjugating enzyme Ubc12 antibody
      • NEDD8 protein ligase antibody
      • NEDD8-conjugating enzyme Ubc12 antibody
      • UBC-RS2 antibody
      • UBC12 antibody
      • UBC12_HUMAN antibody
      • ube2m antibody
      • ubiquitin carrier protein M antibody
      • Ubiquitin conjugating enzyme E2 M antibody
      • Ubiquitin-conjugating enzyme E2 M antibody
      • ubiquitin-protein ligase M antibody
      • yeast UBC12 homolog antibody
      see all

    Anti-UBE2M antibody [EPR5334] images

    • All lanes : Anti-UBE2M antibody [EPR5334] (ab124946) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 21 kDa

    References for Anti-UBE2M antibody [EPR5334] (ab124946)

    ab124946 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124946.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"