Anti-Ube2N / Ubc13 antibody (ab103019)

Overview

  • Product nameAnti-Ube2N / Ubc13 antibody
    See all Ube2N / Ubc13 primary antibodies
  • Description
    Rabbit polyclonal to Ube2N / Ubc13
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Ube2N/ Ubc13.

  • Positive control
    • This antibody gave a positive signal in Human Small Intestine, Mouse Testis and Rat Testis tissue lysates, and the following whole cell lysates: HeLa; HeLa Nuclear; HEK293; U20S; Jurkat; NIH3T3; PC12.

Properties

Applications

Our Abpromise guarantee covers the use of ab103019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

Target

  • FunctionThe UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications
    Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bendless like ubiquitin conjugating enzyme antibody
    • Bendless-like ubiquitin-conjugating enzyme antibody
    • BLU antibody
    • EC 6.3.2.19 antibody
    • Epididymis secretory protein Li 71 antibody
    • HEL-S-71 antibody
    • Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds antibody
    • MGC131857 antibody
    • MGC8489 antibody
    • UBC 13 antibody
    • Ubc13 antibody
    • UbCH ben antibody
    • UbcH-ben antibody
    • UbcH13 antibody
    • UBCHBEN antibody
    • Ube 2N antibody
    • Ube2n antibody
    • UBE2N_HUMAN antibody
    • Ubiquitin carrier protein N antibody
    • Ubiquitin conjugating enzyme E2 N antibody
    • Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13) antibody
    • Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2N antibody
    • Ubiquitin protein ligase N antibody
    • Ubiquitin-conjugating enzyme E2 N antibody
    • Ubiquitin-protein ligase N antibody
    • Yeast UBC13 homolog antibody
    see all

Anti-Ube2N / Ubc13 antibody images

  • All lanes : Anti-Ube2N / Ubc13 antibody (ab103019) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 6 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • All lanes : Anti-Ube2N / Ubc13 antibody (ab103019) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
    Additional bands at : 32 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Ube2N / Ubc13 antibody (ab103019)

ab103019 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

Submitted Sep 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"