Anti-Ube2N / Ubc13 antibody (ab103019)
Key features and details
- Rabbit polyclonal to Ube2N / Ubc13
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-Ube2N / Ubc13 antibody
See all Ube2N / Ubc13 primary antibodies -
Description
Rabbit polyclonal to Ube2N / Ubc13 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Orangutan -
Immunogen
Synthetic peptide corresponding to Human Ube2N/ Ubc13 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab113550) -
Positive control
- This antibody gave a positive signal in Human Small Intestine, Mouse Testis and Rat Testis tissue lysates, and the following whole cell lysates: HeLa; HeLa Nuclear; HEK293; U20S; Jurkat; NIH3T3; PC12.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab103019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
Target
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Function
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. -
Post-translational
modificationsConjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 541130 Cow
- Entrez Gene: 7334 Human
- Entrez Gene: 93765 Mouse
- Entrez Gene: 116725 Rat
- Omim: 603679 Human
- SwissProt: Q0P5K3 Cow
- SwissProt: P61088 Human
- SwissProt: P61089 Mouse
see all -
Alternative names
- Bendless like ubiquitin conjugating enzyme antibody
- Bendless-like ubiquitin-conjugating enzyme antibody
- BLU antibody
see all
Images
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All lanes : Anti-Ube2N / Ubc13 antibody (ab103019) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 6 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes -
All lanes : Anti-Ube2N / Ubc13 antibody (ab103019) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : Testis (Mouse) Tissue Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 4 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Additional bands at: 32 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab103019 has not yet been referenced specifically in any publications.