Overview

  • Product nameAnti-Ube2N / Ubc13 antibody
    See all Ube2N / Ubc13 primary antibodies
  • Description
    Goat polyclonal to Ube2N / Ubc13
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee, Zebrafish, Macaque Monkey, Frog
  • Immunogen

    Synthetic peptide:

    AGPQDSPFEGGT

    conjugated to KLH, corresponding to amino acids 40-51 of Human Ube2N/ Ubc13

  • Positive control
    • Human Thyroid tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab117090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/25000.
WB 1/500 - 1/2000. Predicted molecular weight: 17.1 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionThe UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications
    Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bendless like ubiquitin conjugating enzyme antibody
    • Bendless-like ubiquitin-conjugating enzyme antibody
    • BLU antibody
    • EC 6.3.2.19 antibody
    • Epididymis secretory protein Li 71 antibody
    • HEL-S-71 antibody
    • Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds antibody
    • MGC131857 antibody
    • MGC8489 antibody
    • UBC 13 antibody
    • Ubc13 antibody
    • UbCH ben antibody
    • UbcH-ben antibody
    • UbcH13 antibody
    • UBCHBEN antibody
    • Ube 2N antibody
    • Ube2n antibody
    • UBE2N_HUMAN antibody
    • Ubiquitin carrier protein N antibody
    • Ubiquitin conjugating enzyme E2 N antibody
    • Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13) antibody
    • Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2N antibody
    • Ubiquitin protein ligase N antibody
    • Ubiquitin-conjugating enzyme E2 N antibody
    • Ubiquitin-protein ligase N antibody
    • Yeast UBC13 homolog antibody
    see all

Anti-Ube2N / Ubc13 antibody images

  • ab117090, at 2.5 µg/ml staining UBE2N / Ubc13 in Human Thyroid cells by Immunohistochemistry. Tissue was Formalin-Fixed, Paraffin-Embedded (FFPE) after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with ab117090, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References for Anti-Ube2N / Ubc13 antibody (ab117090)

ab117090 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"